cGK2/PRKG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35308

Novus Biologicals, part of Bio-Techne
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NBP3-35308-100ul
NBP3-35308-20ul
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all cGK2/PRKG2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human cGK2/PRKG2 (NP_006250.1).

Sequence:
MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for cGK2/PRKG2 Antibody

cGK2/PRKG2 Antibody

Western Blot: cGK2/PRKG2 Antibody [NBP3-35308] -

Western Blot: cGK2/PRKG2 Antibody [NBP3-35308] - Western blot analysis of various lysates using cGK2/PRKG2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
cGK2/PRKG2 Antibody

Immunoprecipitation: cGK2/PRKG2 Antibody [NBP3-35308] -

Immunoprecipitation: cGK2/PRKG2 Antibody [NBP3-35308] - Immunoprecipitation analysis of 600 ug extracts of Mouse lung using 3 ug cGK2/PRKG2 antibody. Western blot was performed from the immunoprecipitate using cGK2/PRKG2 antibody at a dilution of 1:1000.

Applications for cGK2/PRKG2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5ug-4ug antibody for 400ug-600ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: cGK2/PRKG2

cGK2/PRKG2, also known as cGMP-dependent protein kinase 2, is 762 amino acids long and approximately 87kDa. cGK2/PRKG2 is a protein kinase that is highly expressed in the brain, intestine and lung. Current research surrounding cGK2/PRKG2 has shown a potential link with several disorders and diseases including cystic fibrosis, gout, malaria, lung cancer, alcoholism, diarrhea, and hyperphenylalaninemia. cGK2/PRKG2 may also interact with PTS, LASP1, YWHAB, MYLK, and PLCB3 in pathways such as cGMP signaling, platelet homeostasis, EDNRB signaling, signaling in gap junctions, nitric oxide metabolism, and eNOS activation and regulation.

Long Name

cGMP-dependent protein kinase 2

Alternate Names

cGK 2, cGK2, cGKII, PRKG2, PRKGR2

Gene Symbol

PRKG2

Additional cGK2/PRKG2 Products

Product Documents for cGK2/PRKG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for cGK2/PRKG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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