CGR19 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85644

Novus Biologicals, part of Bio-Techne
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NBP1-85644
NBP1-85644-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CGR19 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IYDIISMVSVIHIPDRTYKLSCRILYQYLLLAQGQFHDLKQLFMSANNNFTPSNNSSSEEKNTDRSLLEKVGLSESEVEPSEENSKDCVVCQNGTVNWVLLPCRHTCLCDGCVKYFQQCPMCRQFVQESFALCSQKEQDKDKPK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CGR19 Antibody

Western Blot: CGR19 Antibody [NBP1-85644]

Western Blot: CGR19 Antibody [NBP1-85644]

Western Blot: CGR19 Antibody [NBP1-85644] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: CGR19 Antibody [NBP1-85644]

Immunocytochemistry/ Immunofluorescence: CGR19 Antibody [NBP1-85644]

Immunocytochemistry/Immunofluorescence: CGR19 Antibody [NBP1-85644] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CGR19 Antibody [NBP1-85644]

Immunohistochemistry-Paraffin: CGR19 Antibody [NBP1-85644]

Immunohistochemistry-Paraffin: CGR19 Antibody [NBP1-85644] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for CGR19 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CGR19

CGR19 is a gene that codes for a protein that is able to inhibit growth in certain cell lines, which is 332 amino acids long and weighs approximately 38 kDa. CGR19 has been shown to have interactions with UBE2U, CREB3, PEX10, and UBC.

Alternate Names

cell growth regulator with ring finger domain 1, Cell growth regulatory gene 19 protein, CGR19cell growth regulator with RING finger domain protein 1, RING finger protein 197, RNF197GCRRF1

Gene Symbol

CGRRF1

Additional CGR19 Products

Product Documents for CGR19 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CGR19 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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