Skip to main content

CHAF1A Antibody (SS 1 1-13)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-207

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB500-207
NB500-207SS

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SS 1 1-13

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Recombinant human CHAF1A [UniProt# Q13111]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHAF1A Antibody (SS 1 1-13)

Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207]

Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207]

Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/ Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207]

Immunocytochemistry/ Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207]

Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).

Applications for CHAF1A Antibody (SS 1 1-13)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHAF1A

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Alternate Names

CAF, CAF1, CAF-1, CAF-1 subunit A, CAF1B, CAF1P150CAF-I 150 kDa subunit, CAF-I p150, chromatin assembly factor 1 subunit A, chromatin assembly factor 1, subunit A (p150), chromatin assembly factor I (150 kDa), Chromatin assembly factor I p150 subunit, hp150, MGC71229, P150

Entrez Gene IDs

10036 (Human)

Gene Symbol

CHAF1A

UniProt

Additional CHAF1A Products

Product Documents for CHAF1A Antibody (SS 1 1-13)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHAF1A Antibody (SS 1 1-13)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...