CHAF1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74609

Novus Biologicals, part of Bio-Techne
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NB100-74609
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CHAF1A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human chromatin assembly factor 1, subunit A (p150) using the numbering given in entry AAA76736.1 (GeneID 10036).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHAF1A Antibody

Western Blot: CHAF1A Antibody [NB100-74609]

Western Blot: CHAF1A Antibody [NB100-74609]

Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609]

Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609]

Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609] - Human lung cancer. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: CHAF1A Antibody [NB100-74609]

Western Blot: CHAF1A Antibody [NB100-74609]

Western Blot: CHAF1A Antibody [NB100-74609] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74609. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74608 and NB100-74610.
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Applications for CHAF1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHAF1A

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM]

Alternate Names

CAF, CAF1, CAF-1, CAF-1 subunit A, CAF1B, CAF1P150CAF-I 150 kDa subunit, CAF-I p150, chromatin assembly factor 1 subunit A, chromatin assembly factor 1, subunit A (p150), chromatin assembly factor I (150 kDa), Chromatin assembly factor I p150 subunit, hp150, MGC71229, P150

Entrez Gene IDs

10036 (Human)

Gene Symbol

CHAF1A

UniProt

AAA76736.1 (Human)

Additional CHAF1A Products

Product Documents for CHAF1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CHAF1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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