CHAF1B Antibody (OTI4F7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46274

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F7

Concentration

1 mg/ml

View all CHAF1B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CHAF1B(NP_005432) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHAF1B Antibody (OTI4F7)

Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B.
Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274]

Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for CHAF1B Antibody (OTI4F7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200 - 1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHAF1B

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Alternate Names

CAF1, CAF-1, CAF1ACAF-1 subunit B, CAF1P60CAF-I 60 kDa subunit, CAF-IP60, chromatin assembly factor 1 subunit B, chromatin assembly factor 1, subunit B (p60), Chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit, 10, MPHOSPH7CAF-I p60, MPP7M-phase phosphoprotein 7

Gene Symbol

CHAF1B

Additional CHAF1B Products

Product Documents for CHAF1B Antibody (OTI4F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHAF1B Antibody (OTI4F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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