CHAF1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-57523
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies, Tagged Protein Expression, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 509 and the C-terminus (residue 559) of human chromatin assembly factor 1, subunit B (p60) using the numbering given in entry NP_005432.1 (GeneID 8208).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CHAF1B Antibody
This is a test
Western Blot: CHAF1B Antibody [NB100-57523] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-57523 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CAF-1 p60 was also immunoprecipitated by rabbit anti-CAF-1 p60 antibody NB100-57522, which recognizes an upstream epitope. Test![Immunohistochemistry: CHAF1B Antibody [NB100-57523]](https://resources.bio-techne.com/images/products/CHAF1B-Antibody-Immunohistochemistry-NB100-57523-img0004.jpg "Immunohistochemistry: CHAF1B Antibody [NB100-57523]")
Immunohistochemistry: CHAF1B Antibody [NB100-57523]
Immunohistochemistry: CHAF1B Antibody [NB100-57523] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunohistochemistry-Paraffin: CHAF1B Antibody [NB100-57523]](https://resources.bio-techne.com/images/products/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NB100-57523-img0001.jpg "Immunohistochemistry-Paraffin: CHAF1B Antibody [NB100-57523]")
Immunohistochemistry-Paraffin: CHAF1B Antibody [NB100-57523]
Immunohistochemistry-Paraffin: CHAF1B Antibody [NB100-57523] - FFPE section of human seminoma. Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.Applications for CHAF1B Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 mcg/mg
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CHAF1B
Alternate Names
CAF1, CAF-1, CAF1ACAF-1 subunit B, CAF1P60CAF-I 60 kDa subunit, CAF-IP60, chromatin assembly factor 1 subunit B, chromatin assembly factor 1, subunit B (p60), Chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit, 10, MPHOSPH7CAF-I p60, MPP7M-phase phosphoprotein 7
Entrez Gene IDs
8208 (Human)
Gene Symbol
CHAF1B
UniProt
Additional CHAF1B Products
Product Documents for CHAF1B Antibody
Product Specific Notices for CHAF1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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