CHAF1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88235

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88235
NBP1-88235-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHAF1B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PAPTVIRDPPSITPAVKSPLPGPSEEKTLQPSSQNTKAHPSRRVTLNTLQAWSKTTPRRINLTPLKTDTPPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHAF1B Antibody

Western Blot: CHAF1B Antibody [NBP1-88235]

Western Blot: CHAF1B Antibody [NBP1-88235]

Western Blot: CHAF1B Antibody [NBP1-88235] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: CHAF1B Antibody [NBP1-88235]

Immunocytochemistry/ Immunofluorescence: CHAF1B Antibody [NBP1-88235]

Immunocytochemistry/Immunofluorescence: CHAF1B Antibody [NBP1-88235] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235]

Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235]

Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
View 3 more images

Applications for CHAF1B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30423293).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHAF1B

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Alternate Names

CAF1, CAF-1, CAF1ACAF-1 subunit B, CAF1P60CAF-I 60 kDa subunit, CAF-IP60, chromatin assembly factor 1 subunit B, chromatin assembly factor 1, subunit B (p60), Chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit, 10, MPHOSPH7CAF-I p60, MPP7M-phase phosphoprotein 7

Entrez Gene IDs

8208 (Human)

Gene Symbol

CHAF1B

UniProt

Q13112 (Human)

Additional CHAF1B Products

Product Documents for CHAF1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHAF1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.