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CHCHD10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91169

Novus Biologicals, part of Bio-Techne
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NBP1-91169
NBP1-91169-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GLMAQMATTAAGVAVGSAVGHVMGSALTGAFSGGSSEPSQPAVQQAPTPAAPQPLQMGPCAYEIRQFLDCSTTQSDLSLCEGFSEALKQCKYYHGLSSLP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHCHD10 Antibody

Immunohistochemistry-Paraffin: CHCHD10 Antibody [NBP1-91169]

Immunohistochemistry-Paraffin: CHCHD10 Antibody [NBP1-91169]

Immunohistochemistry-Paraffin: CHCHD10 Antibody [NBP1-91169] - Analysis in human skeletal muscle and skin tissues. Corresponding CHCHD10 RNA-seq data are presented for the same tissues.
Western Blot: CHCHD10 Antibody [NBP1-91169]

Western Blot: CHCHD10 Antibody [NBP1-91169]

Western Blot: CHCHD10 Antibody [NBP1-91169] - Analysis in human cell lines PC-3 and U-251MG using anti-CHCHD10 antibody. Corresponding CHCHD10 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: CHCHD10 Antibody [NBP1-91169]

Immunocytochemistry/ Immunofluorescence: CHCHD10 Antibody [NBP1-91169]

Immunocytochemistry/Immunofluorescence: CHCHD10 Antibody [NBP1-91169] - Staining of human cell line A-431 shows positivity in mitochondria. Antibody staining is shown in green.

Applications for CHCHD10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHCHD10

Alternate Names

C22orf16, coiled-coil-helix-coiled-coil-helix domain containing 10, mitochondrial, N27C7-4, protein N27C7-4

Gene Symbol

CHCHD10

Additional CHCHD10 Products

Product Documents for CHCHD10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHCHD10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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