CHD1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89153

Novus Biologicals, part of Bio-Techne
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NBP1-89153
NBP1-89153-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHD1L Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLLEKASQEGRSLRNKGSVLIPGLVEGSTKRKRVLSPEELEDRQKKRQEAAAKRRRLIEEKKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD1L Antibody

Western Blot: CHD1L Antibody [NBP1-89153]

Western Blot: CHD1L Antibody [NBP1-89153]

Western Blot: CHD1L Antibody [NBP1-89153] - Analysis using Anti-CHD1L antibody NBP1-89153 (A) shows similar pattern to independent antibody NBP1-89154 (B).
Immunocytochemistry/ Immunofluorescence: CHD1L Antibody [NBP1-89153]

Immunocytochemistry/ Immunofluorescence: CHD1L Antibody [NBP1-89153]

Immunocytochemistry/Immunofluorescence: CHD1L Antibody [NBP1-89153] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153]

Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153]

Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
View 2 more images

Applications for CHD1L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD1L

In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 (PubMed 19661379)).(supplied by OMIM)

Long Name

Chromodomain Helicase DNA Binding Protein 1-like

Alternate Names

ALC1

Gene Symbol

CHD1L

Additional CHD1L Products

Product Documents for CHD1L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHD1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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