Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CHD3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1575 and 1625 of human chromodomain helicase DNA binding protein 3 using the numbering given in entry NP_001005273.1 (GeneID 1107).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

226 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Please note, this product recently (April 19, 2012) changed concentrations from 0.2 mg/ml to 1.0 mg/ml.

Scientific Data Images for CHD3 Antibody

Western Blot: CHD3 Antibody [NB100-60412]

Western Blot: CHD3 Antibody [NB100-60412]

Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412]

Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412]

Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for CHD3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD3

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Alternate Names

ATP-dependent helicase CHD3, CHD-3, chromodomain helicase DNA binding protein 3, chromodomain-helicase-DNA-binding protein 3, EC 3.6.1, EC 3.6.4.12, hZFH, Mi-2 autoantigen 240 kDa protein, Mi-2a, Mi2-ALPHA, ZFH, Zinc finger helicase, zinc-finger helicase (Snf2-like)

Entrez Gene IDs

1107 (Human)

Gene Symbol

CHD3

UniProt

Q12873 (Human)

Additional CHD3 Products

Product Documents for CHD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHD3 Antibody

Please note, this product recently (April 19, 2012) changed concentrations from 0.2 mg/ml to 1.0 mg/ml.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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