I am working with Chd7 and have just visited your website. I would like to have some advices or protocols to use the NBP1-77393 Ab, especially western blot protocol/extraction buffers. My concern is that Chd7 is a high molecular weight protein and it seems very difficult to extract it and to transfer it on a membrane I was just wondering if you had any tips regarding this matter

Name: CHD7 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-77393
Rating: 5 (1 reviews)

It was a 5% gel ran 100V for 60 min stressing that it was done in cold conditions with ice packs. It should be ok to do overnight at a lower voltage also in cold conditions. We had the best success on NIH3T3 cells, but also tested on Jurkat. In regards to your inquiry about the Western Blot protocol specific for NBP1-77393, we do have one available on our website. You can find this under the protocol tab. In case you have trouble locating this I will provide it to you in this email. These are just suggestions and some additional optimization may need to be done to achieve the best results based on sample preparation and protein expression. Western Blot Protocol: 1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus. 3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate. 4. Rinse the blot. 5. Block the membrane using standard blocking buffer for at least 1 hour. 6. Wash the membrane in wash buffer three times for 10 minutes each. 7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature. 8. Wash the membrane in wash buffer three times for 10 minutes each. 9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. 10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background). 11. Apply the detection reagent of choice in accordance with the manufacturers instructions. Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

CHD7 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77393

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Alexa Fluor 350

NBP1-77393AF350

CHD7 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77393

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