ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43233
![Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]](https://resources.bio-techne.com/images/products/ChemR23-CMKLR1-Antibody-BZ194-Immunohistochemistry-Paraffin-NBP1-43233-img0003.jpg "Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # BZ194
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial synthetic peptide made to an N-terminal portion of the mouse ChemR23/CMKLR1 protein (amino acids 8-32) [UniProt P97468]
Localization
Multi-pass membrane protein with cytoplasmic and extra-cellular domains
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]
Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 dilution. The antibody generated an expected membrane-cytoplasmic staining in the hepatocytes.![Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]](https://resources.bio-techne.com/images/products/ChemR23-CMKLR1-Antibody-BZ194-Flow-Cytometry-NBP1-43233-img0002.jpg "Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]")
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - BSA Free [NBP1-43233]
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - Staining of resident mouse peritoneal macrophages.
Immunohistochemistry-paraffin: Rat Monoclonal ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] -
Immunohistochemistry-paraffin: Rat Monoclonal ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - ChemR23/CMKLR1 was detected in perfusion fixed paraffin-embedded sections of mouse intestine using ChemR23/CMKLR1 Antibody (BZ194) at a dilution of 1:25 for 1 hour at room temperature. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in intestinal villi. Image validated internally.Applications for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:100 - 1:200
Immunohistochemistry-Paraffin
1:100 - 1:200
Application Notes
This antibody was tested in Flow on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P it was tested on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ChemR23
Long Name
Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23
Alternate Names
cmklr1, Dez, Gpcr27
Entrez Gene IDs
14747 (Mouse)
Gene Symbol
CMKLR1
UniProt
Additional ChemR23 Products
Product Documents for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
Product Specific Notices for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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