Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CHIP/STUB1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 253 and 303 of human STIP1 homology and U-box containing protein 1 (carboxy terminus of Hsp70-interacting protein) using the numbering given in entry NP_005852.2 (GeneID 10273).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHIP/STUB1 Antibody

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316] - Section of human breast carcinoma. Antibody: Affinity purified rabbit antiSTUB1/CHIP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: CHIP/STUB1 Antibody [NB100-77316]

Immunoprecipitation: CHIP/STUB1 Antibody [NB100-77316]

Immunoprecipitation: CHIP/STUB1 Antibody [NB100-77316] - Detection of Human STUB1/CHIP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-STUB1/CHIP antibody NB100-77316 used for IP at 3 mcg/mg lysate. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77315, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CHIP/STUB1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHIP/STUB1

STUB1/CHIP is an E3 ubiquitin ligase and co-chaperone of heat-shock protein 70 (Hsp70). STUB1/CHIP interacts with Hsp70 to monitor protein folding and direct misfolded proteins for proteasomal degradation. It has been found to also associate with Hsp90 to remove phosphorylated protein tau, a protein that accumulates in the brains of Alzheimers and Down's syndrome patients. STUB1/CHIP has been reported to also have intrinsic chaperone activity and recognize non-native proteins in response to heat stress. In this way, STUB1/CHIP has been shown to be a direct chaperone for p53.

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Entrez Gene IDs

10273 (Human)

Gene Symbol

STUB1

UniProt

NP_005852.2 (Human)

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHIP/STUB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.