Chk1 Antibody (0S3Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16353

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16353-100ul
NBP3-16353-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0S3Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Chk1 Antibodies »

Product Summary for Chk1 Antibody (0S3Q1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Chk1 (O14757). MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 Antibody (0S3Q1)

Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353]

Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353]

Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353]

Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353]

Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353] - Immunohistochemistry of paraffin-embedded human colon using Chk1 Rabbit mAb (NBP3-16353) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Chk1 Antibody (0S3Q1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Chk1

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 Antibody (0S3Q1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chk1 Antibody (0S3Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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