Chk2 Antibody (CL9440)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88933

Novus Biologicals, part of Bio-Techne
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NBP2-88933
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9440

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Chk2 Antibodies »

Product Summary for Chk2 Antibody (CL9440)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNG

Epitope

QEPEEPTPAPWARLW

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Chk2 Antibody (CL9440)

Western Blot: Chk2 Antibody (CL9440) [NBP2-88933]

Western Blot: Chk2 Antibody (CL9440) [NBP2-88933]

Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933]

Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933]

Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933]

Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933]

Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.
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Applications for Chk2 Antibody (CL9440)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk2

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Gene Symbol

CHEK2

Additional Chk2 Products

Product Documents for Chk2 Antibody (CL9440)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chk2 Antibody (CL9440)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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