Cholecystokinin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18225

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all CCK Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cholecystokinin (Accession P06307), corresponding to amino acid residues I79-R105.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cholecystokinin Antibody

Western Blot: Cholecystokinin Antibody [NBP3-18225]

Western Blot: Cholecystokinin Antibody [NBP3-18225]

Western Blot: Cholecystokinin Antibody [NBP3-18225] - Western blot analysis of Cholecystokinin in NIH-3T3 cell lysate. Samples were incubated with Cholecystokinin polyclonal antibody (Product # NBP3-18225).
Immunocytochemistry/ Immunofluorescence: Cholecystokinin Antibody [NBP3-18225]

Immunocytochemistry/ Immunofluorescence: Cholecystokinin Antibody [NBP3-18225]

Immunocytochemistry/Immunofluorescence: Cholecystokinin Antibody [NBP3-18225] - Immunofluorescent analysis of Cholecystokinin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25C), incubated with mouse anti-beta tubulin and Cholecystokinin polyclonal antibody (Product # NBP3-18225) using a dilution of 1:200 (1 hr, 37C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225]

Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225]

Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP3-18225] - Immunohistochemistry analysis of paraffin-embedded Cholecystokinin in human pancreas tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22C), incubated with Cholecystokinin polyclonal antibody (Product # NBP3-18225) using a dilution of 1:200 (1.5 hr, 22C), followed by HRP conjugated goat anti-rabbit.

Applications for Cholecystokinin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000 - 1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CCK

The regulated translation of messenger RNA is essential for cell-cycle progression, establishment of the body plan during early development and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, one mechanism of controlling translation, is driven by cytoplasmic polyadenylation element binding protein, CPEB. CPEB is a highly conserved, sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element, thereby modulating translational repression and mRNA localization. Blocking cytoplasmic polyadenylation by interfering with the CPE or CPEB prevents the translational activation and translational repression of mRNAs crucial for oocyte maturation. CPEB is synthesized during oogenesis and stockpiled in the oocyte. CPEB degradation occurs via the proteasome pathway, most likely through ubiquitin-conjugated intermediates.

Long Name

Cholecystokinin

Alternate Names

cholecystokinin, MGC117187

Gene Symbol

CCK

Additional CCK Products

Product Documents for Cholecystokinin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cholecystokinin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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