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Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43796

Novus Biologicals, part of Bio-Techne
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NBP3-43796

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # CL3173

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P28329, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

: 2-10 ug/ml

Immunohistochemistry

: 1:500 - 1:1000

Immunohistochemistry-Paraffin

: 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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