Choline Acetyltransferase/ChAT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33530

Novus Biologicals, part of Bio-Techne
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NBP2-33530
NBP2-33530-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Choline Acetyltransferase/ChAT Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human lymph node shows no positivity in lymphoid cells as expected.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of mouse basal forebrain shows strong cytoplasmic positivity in neuronal cells and fibers.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of mouse cerebellum shows positivity in a subset of neural fiber endings.
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Applications for Choline Acetyltransferase/ChAT Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

UniProt

P28329 (Human)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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