CHREBP Antibody (2D9NB) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44307

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D9NB

Format

BSA Free

Concentration

1.0 mg/ml

View all CHREBP Antibodies »

Product Specifications

Immunogen

Partial recombinant human ChREBP protein between amino acids 600-800.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31289120). Use in Rat reported in scientific literature (PMID:19363290).

Localization

Cytoplasm, Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for CHREBP Antibody (2D9NB) - BSA Free

Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunocytochemistry/ Immunofluorescence: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern in the cytoplasm of cancer cells. Some cells depicted nuclear positivity also while the stroma was largely negative for CHREBP expression.
Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) - BSA Free [NBP2-44307]

Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern mainly in the cytoplasm (but in some nuclei also) of cuboidal epithelial cells of various ducts and tubules.

Applications for CHREBP Antibody (2D9NB) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 31289120)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHREBP

Carbohydrate response element-binding protein (ChREBP) is a glucose sensitive basic helix-loop-helix/leucine zipper transcription factor which heterodimerise with MLX and binds to carbohydrate response element (ChoRE) on DNA for the induction genes involved in glycolysis, de novo lipogenesis (DNL), and fatty acid desaturation. Some of the target genes of ChREBP are glucokinase (GCK), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), pyruvate kinase/liver type pyruvate kinase (PK1/ PKLR), delta-9-desaturase (SCD/SCD1) etc. ChREBP is expressed as alpha and beta alternatively spliced isoforms, and among these two isoforms, the alpha-isoform is activated in a glucose-dependent manner. ChREBP is expressed in the cytoplasm and nuclei of a number of mammalian tissues, with high levels found in liver, skeletal muscle, white/brown adipose, heart, kidney, cerebellum and intestine. Glucose stimulates ChREBP transcriptional expression and activates the transactivity of ChREBP protein. It is activated independent of insulin and recent findings have suggested that together with its binding partner Mlx, ChREBP implicates in metabolic processes, insulin signaling related pathways, and also tumorigenesis/cancers. Also, increased hepatic ChREBP expression has been suggested to result in development of non-alcoholic fatty liver disease (NAFLD) and obesity through the conversion of carbohydrates into triglycerides (de novo lipogenesis).

Alternate Names

BHLHD14, CHREBPcarbohydrate-responsive element-binding protein, Class D basic helix-loop-helix protein 14, MIOWS basic-helix-loop-helix leucine zipper protein, MLX interacting protein-like, MLX interactor, MLX-interacting protein-like, MONDOBWilliams-Beuren syndrome chromosomal region 14 protein, WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1, Williams Beuren syndrome chromosome region 14, Williams-Beuren syndrome chromosome region 14 protein 2, WS-bHLHbHLHd14carbohydrate response element binding protein

Entrez Gene IDs

51085 (Human)

Gene Symbol

MLXIPL

UniProt

Q9NP71-1 (Human)

Additional CHREBP Products

Product Documents for CHREBP Antibody (2D9NB) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CHREBP Antibody (2D9NB) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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