Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Monkey

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LK2H10 + PHE5 + CGA/414

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Chromogranin A Antibodies »

Product Specifications

Immunogen

Human pheochromocytoma (LK2H10 & PHE5) & recombinant human chromogranin A protein (CGA414) (Uniprot: P10645)

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34240).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10+PHE5+CGA414)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - Azide and BSA Free [NBP2-34674] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10=PHE5+CGA414)

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34674 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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