Chromogranin A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86056

Novus Biologicals, part of Bio-Techne
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NBP1-86056
NBP1-86056-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Chromogranin A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22412953), Mouse reactivity reported in scientific literature (PMID: 24843046).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chromogranin A Antibody

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056]

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056]

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056] - Staining in human parathyroid gland and liver tissues using anti-CHGA antibody. Corresponding CHGA RNA-seq data are presented for the same tissues.
Western Blot: Chromogranin A Antibody [NBP1-86056]

Western Blot: Chromogranin A Antibody [NBP1-86056]

Western Blot: Chromogranin A Antibody [NBP1-86056] - Analysis in human plasma.
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NBP1-86056]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NBP1-86056]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NBP1-86056] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
View 2 more images

Applications for Chromogranin A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chromogranin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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