CHT1 Antibody (62-2E8)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74570

Novus Biologicals, part of Bio-Techne
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NB110-74570
NB110-74570SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 62-2E8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CHT1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein from the C-terminal portion of the human CHT1 protein. [UniProt# Q9GZV3]

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 12820166)

Localization

Membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CHT1 Antibody (62-2E8)

Western Blot: CHT1 Antibody (62-2E8) [NB110-74570]

Western Blot: CHT1 Antibody (62-2E8) [NB110-74570]

Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570]

Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570]

Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain

Applications for CHT1 Antibody (62-2E8)

Application
Recommended Usage

Immunohistochemistry

1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500
Application Notes
This CHT1 antibody is useful for Immunohistochemistry frozen sections and paraffin embedded sections and Western blot. In Western blot a band can be seen at ~65 kDa representing CHT and a band can also be seen at ~45 kDa after deglycosylation with PNGase F.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHT1

CHT1 (high affinity choline transporter 1) is a member of sodium:solute symporter (SSF) (TC 2.A.21) family that facilitates sodium/chloride-ion dependent import of choline from extracellular space to the neurons with high affinity. CHT1 is expressed in putamen, spinal cord and medulla, and is specific for cholinergic neurons. Acetyl choline is synthesized from choline and acetyl CoA by choline acetyltransferase in the cytoplasm of cholinergic neurons, and then incorporated into synaptic vesicles by VAChT (vesicular acetylcholine transporter). In the pre-synaptic terminals of cholinergic neurons, CHT1 mediates choline uptake which is the rate limiting step for acetyl-choline biosynthesis and is essential for post-natal viability. CHT1 been shown to be internalized in a clathrin dependent manner, whereas, the synaptic vesicles exocytosis enhances its cell surface expression; a mechanism that facilitates CHT1 functions at cellular surfaces with its predominant localization in the synaptic vesicles in cholinergic neurons. Moreover, Nedd4-2-mediated ubiquitination regulates CHT1 cell surface expression reflecting a possibility that treatments or drugs which inhibit CHT1-Nedd4-2 interactions might be useful for diseases involving decrease in acetylcholine level such as Alzheimer's disease. Moreover, polymorphic variation in CHT1 has been shown to predict early, subclinical measures of carotid atherosclerosis which may aid in understanding cholinergic-vagal processes potentially underlying atherosclerotic risk.

Alternate Names

CHT, CHT1MGC126299, hCHT, Hemicholinium-3-sensitive choline transporter, high affinity choline transporter 1, high affinity choline transporter; hemicholinium-3-sensitive cholinetransporter, MGC126300, solute carrier family 5 (choline transporter), member 7, Solute carrier family 5 member 7

Entrez Gene IDs

60482 (Human); 85426 (Rat)

Gene Symbol

SLC5A7

UniProt

Q9GZV3 (Human)

Additional CHT1 Products

Product Documents for CHT1 Antibody (62-2E8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CHT1 Antibody (62-2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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