CHTOP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88085
![Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunohistochemistry-Paraffin-NBP1-88085-img0010.jpg "Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GRGRGMIGRGRGGFGGRGRGRGRGRGALARPVLTKEQLDNQLDAYMSKTKGHLDAELDAYMAQTDPETND
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CHTOP Antibody
![Western Blot: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Western-Blot-NBP1-88085-img0009.jpg "Western Blot: CHTOP Antibody [NBP1-88085]")
Western Blot: CHTOP Antibody [NBP1-88085]
Western Blot: CHTOP Antibody [NBP1-88085] - Analysis in control (vector only transfected HEK293T lysate) and CHTOP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88085-img0007.jpg "Immunocytochemistry/ Immunofluorescence: CHTOP Antibody [NBP1-88085]")
Immunocytochemistry/ Immunofluorescence: CHTOP Antibody [NBP1-88085]
Immunocytochemistry/Immunofluorescence: CHTOP Antibody [NBP1-88085] - Staining of human cell line A-431 shows localization to nuclear speckles & cytoplasmic bodies. Antibody staining is shown in green.![Immunohistochemistry-Frozen: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunohistochemistry-Frozen-NBP1-88085-img0016.jpg "Immunohistochemistry-Frozen: CHTOP Antibody [NBP1-88085]")
Immunohistochemistry-Frozen: CHTOP Antibody [NBP1-88085]
Immunohistochemistry-Frozen: CHTOP Antibody [NBP1-88085] - The staining was done on an E11.5 mouse transverse section (fixed on 4% PFA overnight) and done using standard IF techniques. Antibody at 1:50. Blocking: 1 hour with 1% BSA before addition of the primary antibody. Secondary antibody: Alexa Fluor Donkey Anti Rabbit 488. HC-Fr image submitted by a verified customer review.![Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunohistochemistry-Paraffin-NBP1-88085-img0013.jpg "Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]")
![Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunohistochemistry-Paraffin-NBP1-88085-img0014.jpg "Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]")
![Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]](https://resources.bio-techne.com/images/products/CHTOP-Antibody-Immunohistochemistry-Paraffin-NBP1-88085-img0015.jpg "Immunohistochemistry-Paraffin: CHTOP Antibody [NBP1-88085]")
Applications for CHTOP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25 - 2 ug/mL
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. This CHTOP Antibody is validated for IHC-Fr from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NBP1-88085 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2), 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CHTOP
Alternate Names
C1orf77, chromatin target of PRMT1, DKFZP547E1010, FOPMGC86949, Friend of PRMT1 protein, MGC131924, pp7704, RP1-178F15.2, Small arginine- and glycine-rich protein, small protein rich in arginine and glycine, SRAGchromosome 1 open reading frame 77
Gene Symbol
CHTOP
Additional CHTOP Products
Product Documents for CHTOP Antibody
Product Specific Notices for CHTOP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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