Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15389
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2O4P8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 148-247 of human Chymase/CMA1/Mast Cell Chymase (CMA1) (P23946). GWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]](https://resources.bio-techne.com/images/products/Chymase-CMA1-Mast-Cell-Chymase-Antibody-2O4P8-Western-Blot-NBP3-15389-img0002.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]")
Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]
Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389] - Analysis of extracts of various cell lines, using Chymase/CMA1/Mast Cell Chymase (CMA1) Rabbit mAb (NBP3-15389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]](https://resources.bio-techne.com/images/products/Chymase-CMA1-Mast-Cell-Chymase-Antibody-2O4P8-Immunohistochemistry-Paraffin-NBP3-15389-img0001.jpg "Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]")
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389]
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389] - Human appendix using Chymase/CMA1/Mast Cell Chymase (CMA1) Rabbit mAb (NBP3-15389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Chymase/CMA1
Alternate Names
CMA1, CYH, MCP3P, Mcpt5, MCT1
Gene Symbol
CMA1
Additional Chymase/CMA1 Products
Product Documents for Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
Product Specific Notices for Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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