cIAP-1/HIAP-2 Antibody (2L0W9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15860

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15860-100ul
NBP3-15860-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2L0W9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all cIAP-1/HIAP-2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human cIAP-1/HIAP-2 (Q13490). MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for cIAP-1/HIAP-2 Antibody (2L0W9)

Western Blot: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860]

Western Blot: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860]

Western Blot: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860] - Western blot analysis of extracts of various cell lines, using cIAP-1/HIAP-2 antibody (NBP3-15860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860]

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860]

Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody (2L0W9) [NBP3-15860] - Immunohistochemistry of paraffin-embedded rat ovary using cIAP-1/HIAP-2 antibody (NBP3-15860) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for cIAP-1/HIAP-2 Antibody (2L0W9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: cIAP-1/HIAP-2

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Alternate Names

BIRC2, cIAP1, HIAP-2, MIHB

Gene Symbol

BIRC2

Additional cIAP-1/HIAP-2 Products

Product Documents for cIAP-1/HIAP-2 Antibody (2L0W9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for cIAP-1/HIAP-2 Antibody (2L0W9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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