cIAP-1/HIAP-2 Antibody (PSH02-10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32187

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32187
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH02-10

Concentration

1 mg/ml

View all cIAP-1/HIAP-2 Antibodies »

Product Summary for cIAP-1/HIAP-2 Antibody (PSH02-10)

Immunogen

Recombinant protein within human cIAP-1/HIAP-2 aa 201-618 / 618. (Uniprot: Q13490)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for cIAP-1/HIAP-2 Antibody (PSH02-10)

cIAP-1/HIAP-2 Antibody (PSH02-10)

Western Blot: cIAP-1/HIAP-2 Antibody (PSH02-10) [NBP3-32187]

Western Blot: cIAP-1/HIAP-2 Antibody (PSH02-10) [NBP3-32187] - Western blot analysis of cIAP-1/HIAP-2 on different lysates with Rabbit anti-cIAP-1/HIAP-2 antibody (NBP3-32187) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HT-29 cell lysate
Lane 6: TF-1 cell lysate
Lane 7: A549 cell lysate
Lane 8: HEK-293 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 1 minute 14 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32187) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for cIAP-1/HIAP-2 Antibody (PSH02-10)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: cIAP-1/HIAP-2

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Alternate Names

BIRC2, cIAP1, HIAP-2, MIHB

Gene Symbol

BIRC2

Additional cIAP-1/HIAP-2 Products

Product Documents for cIAP-1/HIAP-2 Antibody (PSH02-10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for cIAP-1/HIAP-2 Antibody (PSH02-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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