cIAP-1/HIAP-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56128
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A small peptide from within amino acids 100 to 200 of human cIAP1 was used as immunogen, GenBank no. gi|2497238.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for cIAP-1/HIAP-2 Antibody
Western Blot: cIAP-1/HIAP-2 Antibody [NB100-56128]
Western Blot: cIAP-1/HIAP-2 Antibody [NB100-56128] - Analysis of cIAP-1/HIAP-2 in various cancer cell lines using this antibody.Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody [NB100-56128]
Immunohistochemistry-Paraffin: cIAP-1/HIAP-2 Antibody [NB100-56128] - Analysis of cIAP-1/HIAP-2 expression in human gliomas from a brain tissue. A. Two tissue cores showing a gemistocytoma, grade II tumor (left) and an anaplastic glioma-glioblastoma, grade IV tumor (right). A1 and A2, higher magnifications of the gemistocytoma. A3, higher magnification of the anaplastic glioma-glioblastoma. Hematoxylin-eosin counterstain. Higher cIAP1 expression is seen in the less malignant tumor (gemistocytoma) than the more malignant anaplastic (glioma-glioblastoma) tumor.Applications for cIAP-1/HIAP-2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:50-1:200
Western Blot
1:1000-1:2000
Application Notes
cIAP1 has 618 amino acids and migrates at ~70 kDa on SDS-PAGE. Observed molecular weights may vary as genes generally produce, by alternative splicing, multiple transcripts all with introns, putatively encoding multiple different protein isoforms. Additionally, proteins may undergo post translational modifications including phosphorylation, glycosylation and cleavage which can result in differential molecular weights. Protein modifications can vary between cell and tissue type, and proteins can migrate as one or multiple bands depending on the isoform expressed as well as post-translational modifications.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: cIAP-1/HIAP-2
Long Name
Cellular Inhibitor of Apoptosis Protein 1
Alternate Names
BIRC2, cIAP1, HIAP-2, MIHB
Entrez Gene IDs
329 (Human)
Gene Symbol
BIRC2
Additional cIAP-1/HIAP-2 Products
Product Documents for cIAP-1/HIAP-2 Antibody
Product Specific Notices for cIAP-1/HIAP-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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