Cingulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89602

Novus Biologicals, part of Bio-Techne
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NBP1-89602
NBP1-89602-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cingulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGLERQLEEKTEECSRLQELLER

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cingulin Antibody

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.
Western Blot: Cingulin Antibody [NBP1-89602]

Western Blot: Cingulin Antibody [NBP1-89602]

Western Blot: Cingulin Antibody [NBP1-89602] - Analysis using Anti-CGN antibody NBP1-89602 (A) shows similar pattern to independent antibody NBP1-89600 (B).
Western Blot: Cingulin Antibody [NBP1-89602]

Western Blot: Cingulin Antibody [NBP1-89602]

Western Blot: Cingulin Antibody [NBP1-89602] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
View 3 more images

Applications for Cingulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cingulin

Alternate Names

cingulin, DKFZp779N1112, KIAA1319

Entrez Gene IDs

57530 (Human)

Gene Symbol

CGN

UniProt

Q9P2M7 (Human)

Additional Cingulin Products

Product Documents for Cingulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cingulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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