Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cip4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human thyroid receptor interacting protein 10 (Cdc42-interacting protein 4) using the numbering given in entry AAM46851.1 (GeneID 9322).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cip4 Antibody

Immunoprecipitation: Cip4 Antibody [NB100-59833]

Immunoprecipitation: Cip4 Antibody [NB100-59833]

Immunoprecipitation: Cip4 Antibody [NB100-59833] - Detection of human TRIP10/CIP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP10/CIP4 antibody NB100-59833 (lot NB100-59833-2) used for IP at 6 ug per reaction. TRIP10/CIP4 was also immunoprecipitated by a previous lot of this antibody (NB100-59833-1) and rabbit anti-TRIP10/CIP4 antibody NB100-59834. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Cip4 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cip4

TRIP10/CIP4 is a multi-functional adapter protein and plays a role in the translocation of Glut4 (glucose transporter) vesicles to the cell surface in response to insulin. TRIP10/CIP4 has also been found to interact with the active GTPases CDC42 and TC10 as well as the Wiskott-Aldrich syndrome protein (WASP), a protein involved in cytoskeletal organization. Recent studies suggest that CIP4 accumulation may play a role in Huntington's disease pathogenesis.

Alternate Names

cdc42-interacting protein 4, CIP4STOT, HSTP, Protein Felic, Salt tolerant protein, salt tolerator, STPTRIP-10, thyroid hormone receptor interactor 10, thyroid receptor interacting protein 10, Thyroid receptor-interacting protein 10, TR-interacting protein 10

Entrez Gene IDs

9322 (Human)

Gene Symbol

TRIP10

UniProt

AAM46851.1 (Human)

Additional Cip4 Products

Product Documents for Cip4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cip4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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