CIRBP Antibody (3V9S7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15745
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3V9S7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (Q14011). MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSYDSYATHNE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CIRBP Antibody (3V9S7)
Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745]
Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745] - Analysis of extracts of various cell lines, using CIRBP antibody (NBP3-15745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Immunocytochemistry/ Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745]
Immunocytochemistry/Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunofluorescence analysis of U-2 OS cells using CIRBP Rabbit mAb (NBP3-15745) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: CIRBP Antibody (3V9S7) [NBP3-15745]
Immunohistochemistry-Paraffin: CIRBP Antibody (3V9S7) [NBP3-15745] - Mouse brain using CIRBP Rabbit mAb (NBP3-15745) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for CIRBP Antibody (3V9S7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CIRBP
Alternate Names
A18 hnRNP, A18HNRNP, CIRPcold-inducible RNA-binding protein, cold inducible RNA binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein, Glycine-rich RNA-binding protein CIRP
Gene Symbol
CIRBP
Additional CIRBP Products
Product Documents for CIRBP Antibody (3V9S7)
Product Specific Notices for CIRBP Antibody (3V9S7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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