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CISH/CIS-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30943

Novus Biologicals, part of Bio-Techne
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NBP1-30943

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CISH/CIS-1 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CISH/CIS-1. The exact sequence is proprietary.

Reactivity Notes

Chicken (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CISH/CIS-1 Antibody

Western Blot: CISH/CIS-1 Antibody [NBP1-30943]

Western Blot: CISH/CIS-1 Antibody [NBP1-30943]

Western Blot: CISH/CIS-1 Antibody [NBP1-30943] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE
Immunocytochemistry/ Immunofluorescence: CISH/CIS-1 Antibody [NBP1-30943]

Immunocytochemistry/ Immunofluorescence: CISH/CIS-1 Antibody [NBP1-30943]

Immunocytochemistry/Immunofluorescence: CISH/CIS-1 Antibody [NBP1-30943] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP1-30943]

Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP1-30943]

Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP1-30943] - Human lung cancer, using CIS antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for CISH/CIS-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CIS-1

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor.

Long Name

Cytokine Inducible SH2-containing Protein

Alternate Names

CISH

Gene Symbol

CISH

Additional CIS-1 Products

Product Documents for CISH/CIS-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CISH/CIS-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.