Cited-2 Antibody (JA22) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-136

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # JA22

Format

BSA Free

Concentration

1.0 mg/ml

View all Cited-2 Antibodies »

Product Specifications

Immunogen

Human CITED 2 protein [UniProt# Q99967]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cited-2 Antibody (JA22) - BSA Free

Western Blot: Cited-2 Antibody (JA22)BSA Free [NB100-136]

Western Blot: Cited-2 Antibody (JA22)BSA Free [NB100-136]

Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunocytochemistry/ Immunofluorescence: Cited-2 Antibody (JA22) - BSA Free [NB100-136]

Immunocytochemistry/ Immunofluorescence: Cited-2 Antibody (JA22) - BSA Free [NB100-136]

Immunocytochemistry/Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) - BSA Free [NB100-136]

Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) - BSA Free [NB100-136]

Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) [NB100-136] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using Cited-2 antibody at 1:500 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Staining was localized to the nucleus.

Applications for Cited-2 Antibody (JA22) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 19319572)

Western Blot

2ug/mL
Application Notes
In Western blot, a band is seen at 29 kDa. Additional bands may be seen at 36 kDa representing p35srj, the alternatively spliced version of CITED2, and at 70 kDa (unknown). Does not work well on mouse tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cited-2

CITED2 (Cbp/p300-interacting transactivator 2; also called MSG-related protein 1/ MRG1 or P35srj) is a CREBBP/EP300-interacting protein which is highly conserved in placental mammals with 95% identity shared between human and mouse protein. CITED2 is a transcriptional co-activator that stabilizes the interactions between DNA-binding proteins and the histone acetyltransferases CBP (CREB -binding protein) as well as p300. It is localized in the nucleus and can be induced upon hypoxic trigger wherein it functions as a negative regulator of HIF1 mediated signaling by competing with HIF1alpha for binding to CBP/p300. Additionally, CITED2 gets induced by several biological stimuli such as cytokines, serum and LPS in different cell type. CITED2 interacts with a diverse array of proteins including SMAD2, SMAD3, LHX1/2, ESR1/2, WT1, EP300, PPARA, TFAP2A, TFAP2B, TFAP2C, HNF4A etc and exerts versatile biological activities. CITED2 is a critical transcriptional cofactor involved in multiple organ development and mice lacking CITED2 die in utero with cardiac and aortic arch malformations, adrenal gland agenesis, small cranial and dorsal root ganglia, exencephaly, and neural crest and left-right patterning defects. Defects in CITED2 have been linked to ventricular septal defect type 2 (VSD2) and atrial septal defect type 8 (ASD8).

Long Name

Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

Alternate Names

Cited2, MRG-1, MSG-related protein 1, P35srj

Entrez Gene IDs

10370 (Human)

Gene Symbol

CITED2

UniProt

Q99967 (Human)

Additional Cited-2 Products

Product Documents for Cited-2 Antibody (JA22) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cited-2 Antibody (JA22) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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