Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Citron Kinase Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human citron (rho-interacting, serine/threonine kinase 21) using the numbering given in entry NP_009105.1 (GeneID 11113).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Citron Kinase Antibody

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740]

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740]

Immunoprecipitation: Citron Kinase Antibody [NBP1-28740] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Citron antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for Citron Kinase Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Citron Kinase

Citron is a dual specificity serine/threonine protein kinase and a putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It is required for KIF14 localization to the central spindle and midbody and may play a role in cytokinesis. Citron also plays an important role in the regulation of cytokinesis and the development of the central nervous system. [Uniprot]

Alternate Names

CITK, citron (rho-interacting, serine/threonine kinase 21), Citron Rho-Interacting Kinase, Citron Rho-Interacting Serine/Threonine Kinase, CRIK, KIAA0949, MCPH17, Rho-interacting Serine/Threonine kinase 21, serine/threonine kinase 21, Serine/threonine-protein kinase 21, STK21

Entrez Gene IDs

11113 (Human)

Gene Symbol

CIT

UniProt

O14578 (Human)

Additional Citron Kinase Products

Product Documents for Citron Kinase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Citron Kinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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