Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CKAP2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 633 and 683 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP2 Antibody

Western Blot: CKAP2 Antibody [NBP1-21391]

Western Blot: CKAP2 Antibody [NBP1-21391]

Western Blot: CKAP2 Antibody [NBP1-21391] - Detection of human CKAP2 by western blot. Samples:Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKAP2 antibody NBP1-21391 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CKAP2 Antibody [NBP1-21391]

Immunoprecipitation: CKAP2 Antibody [NBP1-21391]

Immunoprecipitation: CKAP2 Antibody [NBP1-21391] - Detection of human CKAP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKAP2 antibody NBP1-21391 used for IP at 3 ug per reaction. CKAP2 was also immunoprecipitated by rabbit anti-CKAP2 antibody NBP1-21390. For blotting immunoprecipitated CKAP2, NBP1-21391 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CKAP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CKAP2

CKAP2 (cytoskeletal-associated protein 2), also known as TMAP (tumor-associated microtubule-associated protein), was identified as an mRNA up-regulated in primary human gastric cancers. It was found to localize to microtubule organizing centers and microtubules and play a role in cell division. CKAP2 is required for proper chromosome segregation and the maintenance of genomic stability. It has recently been shown to be a substrate of Cdk1-cyclinB and essential for bipolar spindle formation.

Alternate Names

CTCL tumor antigen se20-10, cytoskeleton associated protein 2, cytoskeleton-associated protein 2, LB1DKFZp686L1238, se20-10, TMAPFLJ10749, Tumor- and microtubule-associated protein, tumor-associated microtubule-associated protein

Entrez Gene IDs

26586 (Human)

Gene Symbol

CKAP2

UniProt

NP_001091995.1 (Human)

Additional CKAP2 Products

Product Documents for CKAP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CKAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.