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CKAP4/p63 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85571

Novus Biologicals, part of Bio-Techne
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NBP1-85571
NBP1-85571-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CKAP4/p63 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP4/p63 Antibody

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85571 (A) shows similar protein distribution across tissues to independent antibody NBP1-85572 (B).
Western Blot: CKAP4/p63 Antibody [NBP1-85571]

Western Blot: CKAP4/p63 Antibody [NBP1-85571]

Western Blot: CKAP4/p63 Antibody [NBP1-85571] - Analysis in human cell lines U-251MG and MCF-7 using Anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Applications for CKAP4/p63 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKAP4/p63

Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.

Long Name

Cytoskeleton-associated Protein-4/Climp-63

Alternate Names

CLIMP-63, ERGIC-63, p63

Gene Symbol

CKAP4

Additional CKAP4/p63 Products

Product Documents for CKAP4/p63 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CKAP4/p63 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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