Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CKII alpha prime polypeptide Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 350) of human Casein Kinase 2, alpha 2 using the numbering given in entry NP_001887.1 (GeneID 1459).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CKII alpha prime polypeptide Antibody

Western Blot: CKII alpha prime polypeptide Antibody [NB100-379]

Western Blot: CKII alpha prime polypeptide Antibody [NB100-379]

Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379]

Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379]

Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody NB100-378. For blotting immunoprecipitated CKII alpha', NB100-379 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CKII alpha prime polypeptide Antibody

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg lysate

Western Blot

1:200- 1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~40 kDa, representing CKII alpha'.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CKII alpha prime polypeptide

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Long Name

Casein kinase II subunit alpha'

Alternate Names

CK II alpha', CK2A2, CSNK2A2

Entrez Gene IDs

1459 (Human)

Gene Symbol

CSNK2A2

UniProt

P19784 (Human)

Additional CKII alpha prime polypeptide Products

Product Documents for CKII alpha prime polypeptide Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CKII alpha prime polypeptide Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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