CKII alpha prime polypeptide Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99686

Novus Biologicals, part of Bio-Techne
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NBP2-99686-100ul
NBP2-99686-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CKII alpha prime polypeptide Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CKII alpha prime polypeptide (Accession#: NP_001887.1; Met1-Arg350)

Specificity

Human CSNK2A2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CKII alpha prime polypeptide Antibody

Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686]

Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686]

Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686]

Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686]

Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686] - Immunofluorescence staining of CKII alpha prime polypeptide in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CKII alpha prime polypeptide polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686]

Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686]

Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686] - CKII alpha prime polypeptide was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-CKII alpha prime polypeptide rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CKII alpha prime polypeptide rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa

Applications for CKII alpha prime polypeptide Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKII alpha prime polypeptide

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Long Name

Casein kinase II subunit alpha'

Alternate Names

CK II alpha', CK2A2, CSNK2A2

Gene Symbol

CSNK2A2

Additional CKII alpha prime polypeptide Products

Product Documents for CKII alpha prime polypeptide Antibody

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Certificate of Analysis

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Product Specific Notices for CKII alpha prime polypeptide Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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