Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07569

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Monkey, Xenopus

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CHC/1432

Concentration

0.2 mg/ml

View all Clathrin Heavy Chain 1/CHC17 Antibodies »

Product Summary for Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

Immunogen

Recombinant full-length human Clathrin Heavy Chain 1/CHC17 protein

Reactivity Notes

African Green Monkey

Localization

Cytoplasmic

Specificity

Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08333)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

Immunocytochemistry/ Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432) [NBP3-07569]

Immunocytochemistry/ Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432) [NBP3-07569]

Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432) [NBP3-07569] - Confocal Immunofluorescence image of HeLa cells stained with Clathrin Heavy Chain 1/CHC17Monoclonal Antibody (CHC/1432) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuceli red.

Applications for Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Clathrin Heavy Chain 1/CHC17

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Long Name

Clathrin Heavy Chain on Chromosome 17

Alternate Names

CHC17, CLH17, CLTC, CLTCL2

Gene Symbol

CLTC

UniProt

P53675, Q00610 (Human)

Additional Clathrin Heavy Chain 1/CHC17 Products

Product Documents for Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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