Clathrin light chain Antibody (CON.1) - BSA Free

Unconjugated

Monoclonal Mouse IgG_2B Clone # CON.1

BSA Free

2.4 mg/ml

Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain.

Cross-reacts S. cerevisiae. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Cell Membrane and Cytoplasmic

Monoclonal Anti-Clathrin Light Chain reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. The product is useful in immunoblotting 2-4 (two doublets at 25-36 kDa, a doublet at approximately 56 kDa, and possibly also additional lower and higher molecular weight bands), immunoprecipitation, immunocytochemistry (4% paraformaldehyde- 0.5% Triton X-100 fixation), and immunoaffinity purification. Cross-reactivity has been observed with human, bovine, rat, mouse, and yeast.

Monoclonal

Mouse

IgG_2B

Protein A or G purified

10mM PBS (pH 7.4)

BSA Free

0.9% Sodium Azide

2.4 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C. Avoid freeze-thaw cycles.