Clathrin light chain Antibody (SPM174) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54435
![Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) - Azide and BSA Free [NBP2-54435]](https://resources.bio-techne.com/images/products/Clathrin-light-chain-Antibody-SPM174-Immunohistochemistry-Paraffin-NBP2-54435-img0001.jpg "Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) - Azide and BSA Free [NBP2-54435]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM174
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant N-terminal fragment of human Clathrin Light Chain
Localization
Cytoplasmic
Specificity
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53232).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Clathrin light chain Antibody (SPM174) - Azide and BSA Free
Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) - Azide and BSA Free [NBP2-54435]
Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) [NBP2-54435] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).Applications for Clathrin light chain Antibody (SPM174) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Clathrin light chain
Alternate Names
clathrin light chain A, clathrin, light chain A, Lca, light polypeptide (Lca)
Gene Symbol
CLTA
Additional Clathrin light chain Products
Product Documents for Clathrin light chain Antibody (SPM174) - Azide and BSA Free
Product Specific Notices for Clathrin light chain Antibody (SPM174) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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