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Clathrin light chain + heavy chain Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69194

Novus Biologicals, part of Bio-Techne
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NBP1-69194

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to CLTA (clathrin, light chain (Lca)) The peptide sequence was selected from the C terminal of CLTA. Peptide sequence KELEEWYARQDEQLQKTKANNRAAEEAFVNDIDESSPGTEWERVARLCDF. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Clathrin light chain + heavy chain Antibody

Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-69194]

Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-69194]

Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-69194] - Human Brain lysate, concentration 0.2-1 ug/ml.
Immunohistochemistry: Clathrin light chain + heavy chain Antibody [NBP1-69194]

Immunohistochemistry: Clathrin light chain + heavy chain Antibody [NBP1-69194]

Immunohistochemistry: Clathrin light chain + heavy chain Antibody [NBP1-69194] - Human Adult Prostate Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.

Applications for Clathrin light chain + heavy chain Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Clathrin light chain + heavy chain

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12.

Alternate Names

clathrin light chain A, clathrin, light chain A, LCA, light polypeptide (Lca)

Gene Symbol

CLTA

Additional Clathrin light chain + heavy chain Products

Product Documents for Clathrin light chain + heavy chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Clathrin light chain + heavy chain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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