Claudin-1 Antibody (1F8U5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15397

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15397-100ul
NBP3-15397-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F8U5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Claudin-1 Antibodies »

Product Summary for Claudin-1 Antibody (1F8U5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 112-211 of human Claudin-1 (O95832). EVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-1 Antibody (1F8U5)

Western Blot: Claudin-1 Antibody (1F8U5) [NBP3-15397]

Western Blot: Claudin-1 Antibody (1F8U5) [NBP3-15397]

Western Blot: Claudin-1 Antibody (4L4Q2) [NBP3-15397] - Western blot analysis of extracts of various cell lines, using Claudin-1 Rabbit mAb (NBP3-15397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for Claudin-1 Antibody (1F8U5)

Application
Recommended Usage

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-1

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Gene Symbol

CLDN1

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody (1F8U5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Claudin-1 Antibody (1F8U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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