Claudin-1 Antibody (PS01-43)

Western Blot: Claudin-1 Antibody (PS01-43) [NBP3-32189]

Western Blot: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32189) at 1/1,000 dilution.

Lane 1: A431 cell lysate, 10 ug/Lane
Lane 2: HepG2 cell lysate, 10 ug/Lane

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32189) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189]

Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Claudin-1 with Rabbit anti-Claudin-1 antibody (NBP3-32189) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32189, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189]

Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Claudin-1 antibody (NBP3-32189) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32189) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.