Claudin-3 Antibody (OTI1E7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70445

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Claudin-3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CLDN3 (NP_001297) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-3 Antibody (OTI1E7) - Azide and BSA Free

Western Blot: Claudin-3 Antibody (OTI1E7)Azide and BSA Free [NBP2-70445]

Western Blot: Claudin-3 Antibody (OTI1E7)Azide and BSA Free [NBP2-70445]

Western Blot: Claudin-3 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70445] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Claudin-3.
Immunohistochemistry: Claudin-3 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70445]

Immunohistochemistry: Claudin-3 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70445]

Immunohistochemistry: Claudin-3 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70445] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Claudin-3 Antibody (OTI1E7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-3

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1

Gene Symbol

CLDN3

Additional Claudin-3 Products

Product Documents for Claudin-3 Antibody (OTI1E7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Claudin-3 Antibody (OTI1E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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