Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71416

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Claudin-6 Antibodies »

Product Summary for Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human CLDN6(NP_067018) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

Western Blot: Claudin-6 Antibody (OTI2H1)Azide and BSA Free [NBP2-71416]

Western Blot: Claudin-6 Antibody (OTI2H1)Azide and BSA Free [NBP2-71416]

Western Blot: Claudin-6 Antibody (OTI2H1) - Azide and BSA Free [NBP2-71416] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Claudin-6.
Immunocytochemistry/ Immunofluorescence: Claudin-6 Antibody (OTI2H1) - Azide and BSA Free [NBP2-71416]

Immunocytochemistry/ Immunofluorescence: Claudin-6 Antibody (OTI2H1) - Azide and BSA Free [NBP2-71416]

Immunocytochemistry/Immunofluorescence: Claudin-6 Antibody (OTI2H1) - Azide and BSA Free [NBP2-71416] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Claudin-6.

Applications for Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-6

The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-6 is expressed in differentiated F9 cells that resemble tight junction-bearing viceral endoderm resulting from stimulation with retinoc acid and mediated by RXRalpha and RARgamma. Claudin-6 is absent in mouse brain and lung. The human claudin-6 gene maps to chromosome 16p13.3.

Alternate Names

Claudin6, CLDN6, Skullin 2

Gene Symbol

CLDN6

Additional Claudin-6 Products

Product Documents for Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Claudin-6 Antibody (OTI2H1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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