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CLC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19580

Novus Biologicals, part of Bio-Techne
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NBP2-19580

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLC. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLC Antibody

Western Blot: CLC Antibody [NBP2-19580]

Western Blot: CLC Antibody [NBP2-19580]

Western Blot: NR6/CLC Antibody [NBP2-19580] - A. 30 ug 293T whole cell lysate/extractB. 30 ug whole cell lysate/extract of human CLCF1-transfected 293T cells 12% SDS-PAGE NNT-1/BSF-3 antibody dilution: 1:5000.
Immunocytochemistry/ Immunofluorescence: CLC Antibody [NBP2-19580]

Immunocytochemistry/ Immunofluorescence: CLC Antibody [NBP2-19580]

Immunocytochemistry/Immunofluorescence: NR6/CLC Antibody [NBP2-19580] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: CLC Antibody [NBP2-19580]

Immunohistochemistry-Paraffin: CLC Antibody [NBP2-19580]

Immunohistochemistry-Paraffin: NR6/CLC Antibody [NBP2-19580] - Human colon carcinoma, using NNT-1/BSF-3 antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for CLC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CLC

CLCF1 belongs to the interleukin-6 (IL6; MIM 147620) family of cytokines, which are involved in cell signaling through phosphorylation of gp130 (IL6ST; MIM 600694). IL6 family members share similarity in gene structure and have a 4-helix bundle in their protein structure.[supplied by OMIM]

Long Name

Cardiotrophin-like Cytokine

Alternate Names

BSF-3, CISS2, CLCF1, NNT-1, NR6

Gene Symbol

CLCF1

UniProt

Additional CLC Products

Product Documents for CLC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CLC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.