CLCC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06071

Novus Biologicals, part of Bio-Techne
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NBP3-06071-100ul
NBP3-06071-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CLCC1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CLCC1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CLCC1 Antibody

Western Blot: CLCC1 Antibody [NBP3-06071]

Western Blot: CLCC1 Antibody [NBP3-06071]

Western Blot: CLCC1 Antibody [NBP3-06071] - Anti-CLCC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: CLCC1 Antibody [NBP3-06071]

Immunoprecipitation: CLCC1 Antibody [NBP3-06071]

Immunoprecipitation: CLCC1 Antibody [NBP3-06071] - CLCC1 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-CLCC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-CLCC1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size :62 kDa

Applications for CLCC1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLCC1

Chloride channels (CLCs) regulate cellular traffic of chloride ions, a critical component of all living cells. CLCs are involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport. CLCC1 (Chloride channel CLIC-like protein 1), also known as MCLC (Mid-1-related chloride channel) or KIAA0761, is a 551 amino acid multi-pass membrane protein that belongs to the chloride channel MCLC family. CLCC1 is related to the Saccharomyces cerevisiaeprotein Mid-1 and is believed to function as an intracellular chloride channel that is expressed in lung, brain, muscle, liver and testis. Localizing to intracellular compartments such as the Golgi apparatus, the endoplasmic reticulum (ER) and the nuclear envelope, CLCC1 is expressed as four isoforms due to alternative splicing events, namely hMCLC-1, hMCLC-2, hMCLC-3 and hMCLC-4.

Alternate Names

chloride channel CLIC-like 1, KIAA0761, MCLCchloride channel CLIC-like protein 1, Mid1-related chloride channel, Mid-1-related chloride channel 1, Mid-1-related chloride channel protein 1

Gene Symbol

CLCC1

Additional CLCC1 Products

Product Documents for CLCC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CLCC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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