CLEC14A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30854
![Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Immunohistochemistry-Paraffin-NBP2-30854-img0008.jpg "Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ARWDKLSGDVLCPCPGRYLRAGKCAELPNCLDDLGGFACECATGFELGKDGRSCVTSGEGQPTLGGTGVPTRRPPATATSPVPQRTWPIRVDEKL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CLEC14A Antibody - BSA Free
![Western Blot: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Western-Blot-NBP2-30854-img0006.jpg "Western Blot: CLEC14A Antibody [NBP2-30854]")
Western Blot: CLEC14A Antibody [NBP2-30854]
Western Blot: CLEC14A Antibody [NBP2-30854] - Analysis in control (vector only transfected HEK293T lysate) and CLEC14A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30854-img0005.jpg "Immunocytochemistry/ Immunofluorescence: CLEC14A Antibody [NBP2-30854]")
Immunocytochemistry/ Immunofluorescence: CLEC14A Antibody [NBP2-30854]
Immunocytochemistry/Immunofluorescence: CLEC14A Antibody [NBP2-30854] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Immunohistochemistry-Paraffin-NBP2-30854-img0011.jpg "Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]")
Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]
Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854] - Staining of human liver shows low positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Immunohistochemistry-Paraffin-NBP2-30854-img0009.jpg "Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]")
![Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]](https://resources.bio-techne.com/images/products/CLEC14A-Antibody-Immunohistochemistry-Paraffin-NBP2-30854-img0010.jpg "Immunohistochemistry-Paraffin: CLEC14A Antibody [NBP2-30854]")
Applications for CLEC14A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLEC14A
CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain and about which little is known. Mature human CLEC14A is 469 amino acids in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.
Long Name
C-type Lectin Domain Family 14, Member A
Alternate Names
CEG1, EGF R5
Gene Symbol
CLEC14A
UniProt
Additional CLEC14A Products
Product Documents for CLEC14A Antibody - BSA Free
Product Specific Notices for CLEC14A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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