CLEC4E Antibody (16E3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49311

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 16E3

Format

BSA Free

Concentration

1 mg/ml

View all CLEC4E Antibodies »

Product Specifications

Immunogen

Recombinant human CLEC4E (41-219aa) purified from E. coli (NP_055173).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31725411).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLEC4E Antibody (16E3) - BSA Free

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Hepatic mincle (CLEC4E) in cirrhosis and ACLF. Representative Western blot showing protein levels of mincle in control, cirrhotic induced by bile-duct ligation (BDL), and rats with acute-on-chronic liver failure (ACLF) induced by lipopolysaccharide (LPS) within cirrhosis and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunocytochemistry/ Immunofluorescence: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunocytochemistry/Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Applications for CLEC4E Antibody (16E3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:250-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:250-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLEC4E

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor ? chain (FcR?) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Entrez Gene IDs

26253 (Human)

Gene Symbol

CLEC4E

UniProt

Q9ULY5

Additional CLEC4E Products

Product Documents for CLEC4E Antibody (16E3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CLEC4E Antibody (16E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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