CLIC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00172

Novus Biologicals, part of Bio-Techne
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NBP1-00172
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Rat

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CLIC4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence NGLKEEDKEPLIE-C corresponding to N-Terminus according to NP_039234.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CLIC4 Antibody

Western Blot: CLIC4 Antibody [NBP1-00172]

Western Blot: CLIC4 Antibody [NBP1-00172]

Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody [NBP1-00172]

Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody [NBP1-00172]

Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.
Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CLIC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Peptide ELISA

detection limit 1:64000

Western Blot

0.1-0.3 ug/ml
Application Notes
IF: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. WB: Approx. 30 kDa band observed in human kidney and human placenta lysates is reported in scientific literature (PMID: 10070163).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLIC4

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Alternate Names

chloride intracellular channel 4, chloride intracellular channel 4 like, chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, Intracellular chloride ion channel protein p64H1, MTCLIC, p64H1

Entrez Gene IDs

25932 (Human); 29876 (Mouse); 83718 (Rat)

Gene Symbol

CLIC4

UniProt

NP_039234.1 (Human)

Additional CLIC4 Products

Product Documents for CLIC4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CLIC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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